急性早幼粒细胞白血病患者骨髓端粒酶活性测定

Determination of the Activity of Bone Marrow Telomerase in Patients with Acute Promyelocytic Leukemia

[目的]了解急性早幼粒细胞白血病(APL)患者骨髓端粒酶的活性及PML/RARα融合基因表达与端粒酶活性的关系。[方法]20例APL患者(其中8例为发病期 ,12例为缓解期)和10例正常对照者的骨髓标本 ,分离单个核细胞 ;RT_PCR法测PML/RARα融合基因表达 ,TRAP法测端粒酶活性。[结果]8例发病期62 5 %(5/8)端粒酶阳性 ,缓解组和阴性对照组均有1例端粒酶阳性 ;发病的8例PML/RARα融合基因均表达 ,其中5例端粒酶活性亦阳性 ,一致率62 5%。[结论]APL发病期端粒酶活性可能被激活 ,其机理不清 ;端粒酶活性对APL诊断的作用 ,其活性高低是否与预后有关 。

To investigate the telomerase activity in bone marrow of APL patients and the relationship between fusion gene PML/RARα expression and telomerase activity in APL. RT_PCR was used to detect fusion gene PML/RARα expression and TRAP was used to detect telomerase activity in bone marrow in 20 samples of APL patients (8 in occurrent stage,12 in remission stage)and 10 samples of normal people as control.Telomerase activity positive rate was 62 5%(5/8) in occurrent stage of APL;only one positive in remission stage of APL and one in control.Five samples with telomerase activity were positive among occurrent stage patients with fusion gene PML/RARα expression,the coincidence rate was 62 5%.[Conclusion]Telomerase activity may be active in occurrent stage of APL,but its mechanism is unknown.The diagnostic and prognostic significance of the telomerase activity in APL should be further studied.