电脉冲化疗对人甲状腺癌裸鼠移植瘤的作用

Effect of Electrochemotherapy on Thyroid Cancer Cell Line TA K in Nude Mice

[目的]探讨电脉冲化疗对人甲状腺未分化癌裸鼠皮下移植瘤的抗肿瘤效果。[方法]利用人甲状腺未分化癌细胞系TA K进行裸鼠皮下接种 ,建立裸鼠皮下移植瘤模型进行电脉冲化疗实验 ,观察肿瘤局部注射给药和腹腔给药与电脉冲相结合治疗后肿瘤大体和病理组织学变化。[结果]局部注射平阳霉素(PYM)结合电脉冲组与其他实验组比较有明显的抗肿瘤效果 ,其完全缓解率为80 % ,部分缓解率为20 % ,总有效率为100 % ;而腹腔注射PYM结合电脉冲组治疗后肿瘤缩小不足50 % ,肿瘤体积变化与其对照组相比差别不显著 ;对照组、单纯给药组、单纯给予电脉冲组肿瘤继续进展。[结论]利用PYM介导的电脉冲化疗治疗人甲状腺未分化癌裸鼠皮下移植瘤 ,其中局部给药与电脉冲结合效果最好 ;电脉冲化疗无明显局部及全身反应 。

To determine whether pingyangmycin (bleomycin A5) is a better choice for electrochemotherapy on human anaplastic thyroid cancer cell line TA K. Anti tumor efficacy of electrochemotherapy was evaluated on human anaplastic thyroid cancer cell line TA K in vivo.The animal model of ectopic implantation was used.Animals were followed up on periodic tumor volume to determine the response.Histologic change was analysed. A single electrochemotherapy treatment protocol for small tumors resulted in a 100% response rate (CR,80%;PR,20%),tumor progressed in the groups of control,electric pulsed,or drug administration alone.[Conclusion]Electrochemotherapy with bleomycin may have great promise for treatment of human anaplastic thyroid cancer.