p21^(WAF1/CIP1)在甲状腺癌组织的表达及临床意义

Expression and Clinical Significance of p21^(WAF1/CIP1) in Thyroid Carcinoma

[目的]探讨 p21WAF1/CIP1与 p53基因在甲状腺癌中的表达与病理类型和分化程度的关系。[方法]应用免疫组化技术检测甲状腺癌组织中的抑癌基因 p21WAF1/CIP1及 p53基因的表达。统计学采用 χ2 检验。[结果]甲状腺腺瘤(TT) ,甲状腺乳头状癌(PTC)、滤泡状癌(FTC)、未分化癌(UDC)组织 p21蛋白阳性率分别为66 7%、62 5 %、58 3 %、50 0% ,各组之间无显著差别(P>0 05) ;高分化癌(WDC)、低分化癌(PDC)、UDC组织p21蛋白阳性率分别为67 7 %、46 2 %、50 0 % ,各组之间无显著差别(P>0 05) ;PTC、FTC、UDC组织 p53蛋白阳性率分别为31 3%、25 0 %、64 3 % ,UDC与PTC、FTC之间存在显著差别(P<0 05) ;WDC、PDC、UDC组织 p53蛋白阳性率分别为16 1 %、61 5 %、64 3 % ,WDC的阳性率明显低于PDC、UDC(P<0 05) ;p53蛋白阳性的癌组织 p21蛋白阳性率为40 9%(9/22) ,p53蛋白阴性的癌组织 p21蛋白阳性率为69 4%(25/36) ,两者之间存在显者差异(P<0 05)。[结论]甲状腺癌中 p21WAF1/CIP1的表达与其病理类型和分化程度无关 ,而 p53基因的表达与甲状腺癌病理类型和分化程度有关 ,p21WAF1/CIP1作为一新的抑癌基因 ,其表达相当程度上依赖于 p53蛋白。

To investigate expression of p21WAF1/CIP1,p53 gene and their relationship with pathologic type and grade in thyroid carcinoma.Expression of p21WAF1/CIP1 and p53 in thyroid carcinoma was studied by immunohistochemical technology,and analysed by χ2 test.The positive rates of p21 proteins were 62.5%,58.2%,50.0% in the papillary thyroid carcinoma (PTC),follicular thyroid carcinoma(FTC),and undifferentiated carcinoma(UDC) respectively,with no significant difference (P>0 05) to those of well differentiated carcinoma(WDC,67.7%),poor differentiated carcinoma (PDC,46.2%) and UDC(53.0%).The positive rates of p53 proteins in the PTC,FTC,UDC were 31.3%,25.0%,64.3% respectively.The expression of p53 gene in the UDC were significantly higher than that of PTC and FTC (P<0 05).The expression of p53 gene in the UDC (64.3%) and PDC(61.5%) was significantly higher than that in the WDC (16.5%)(P<0.05).The positive rate of p21 protein (40.9%) in the p53 positive samples was significantly lower than that in p53 negative samples (69.4%)(P<0 05).[Conclusion]The p21WAF1/CIP1 is a suppressive gene of tumor,and its expression greatly depends on the p53.