纤维联接蛋白的析出与大鼠皮肤早期损伤的时间关系

THE RELATIONSHIP OF FIBRONECTIN DETECTION AND TIMING OF EARLY RAT'S SKIN WOUNDS

采用免疫组化(PAP)方法,检测大鼠皮肤损伤区的FN,发现在生前创伤后15min,创壁FN即呈明确阳性;随着伤后经历时间的延长,创壁FN逐渐增多,并滑创壁呈条带状沉积;而在死后5min的创伤,创壁FN则呈阴性。本文为区别生前与死后皮肤创伤提出了一种新的方法。

Immunohistochemical PAP method was used to detect the fibronectin (FN) in the rat's skin incised wounds. The results demonstrated that FN manifest itself in the wall of the wounds after 15 min injury, within 1 to 8 hrs after injury, FN accumulated numerously, forming a gradient deposition. In contrast, no FN was found in wounds formed 5 min after death. The research had eatablished a new method for differentiation timing of early wound, and for differentiation of antemortom from postmortem wounds