高温下Bapta四酸甲酯对人红细胞的影响

Influence of Bapta Tetramethyl Ester on Human Erythrocyte Exposed to Hyperthermia

Bapta[1,2-双(邻一氨基酚氧基)乙烷-N,N,N',N'-四乙酸]酯可以透过细胞膜,近年来已成为研究细胞Ca^(2+)生理功能的有力工具.本文以含1mMBapta四酸甲酯的人红细胞悬液置于不同温度中保温60分钟,采用扫描电镜观察细胞形态变化,并测定细胞溶血作用,膜结合Na·K-ATP酶活力变化,分析不同温度下Bapta四酸甲酯对人红细胞的影响.实验结果表明,人红细胞形态和溶血作用实验组与对照组的变化相似,而Na·K-ATP酶活力变化实验组的均比对照组的低,其变化最大的为45℃高温处理组,对照组酶活力最高值为50℃处理组.实验结果看出,1mMBapta四酸甲脂对人红细胞无明显的破坏作用.

Bapta buffers can control calcium outside or inside cells.They can be introduced into cells as permeant tetramethyl ester.Erythrocyte samples in 1 mM Bapta tetramethyl ester solution were incubated at various temperatures for 60 min.This study shows that changes in cell deformation and heamolisis in the control preparation and Bapta tetramethyl ester preparation are the same at various temperature.When human erytrocytes are treated with Bapta tetramethyl ester the activity of Na·Ka-ATPase goes through a sharp maximum centered at about 45℃, while the activity in control centered at 50℃.The present investigation indicates that it is not toxic to human erythrocyte at cytosotic concentrations above about 1 mM.