检测青霉素过敏性休克机体血中IgE类抗体

DETECTION OF IgE ANTIBODIES IN ANIMALS DIED OF ANAPHYLACTIC SHOCK

采用酶联免疫吸附试验(ELISA)法,检测青霉素过敏性休克豚鼠体内的IgE类抗体。结果显示:休克组血清中总IgE的量明显高于对照组(P<0.01)。用青霉噻唑蛋白(Penicilloylated Protein)作包被抗原,检测特异性IgE,以阴性参考血清的平均光密度加3个标准差作为阳性判断标准,发现过敏性休克动物血清中特异性IgE均呈阳性反应。本研究还发现:休克组动物在休克前、后特异性IgE间的差异具有高度显著性(P<0.01),虽然休克后特异性IgE略下降,但仍明显高于阳性标准。本研究认为:IgE量的变化与发生过敏性休克的关系应是:体内IgE升高并不必然导致过敏性休克,但过敏性休克的发生甚至导致死亡,应是IgE,特别是特异性IgE出现和升高的结果。

This paper reports the detection of IgE antibodies in animals died of anaphylactie shock by enzyme--linked immunosorbent assay (ELISA). The total IgE level in serum of shock group was significantly higher than that of control group(P<0.01). The ELISA method specific igE was carried out by coating the plate with penicilloylated protein. Taking the mean optical density(O. D. ) of negative serum control plus three standard deviations as positive standard, the results showed, the specific IgE in every animal died of anaphylactic shock gave positive reaction. It was also found that in the test group the difference of O. D. value was significant different before and after shock. It was believed that the increase of IgE in serum would not inevitablely result in anaphylaetie shock, but the anaphytactic shock was a result of elevation of serum IgE level, especially specific IgE.