细胞周期素依赖激酶抑制因子p16表达与神经母细胞瘤发生发展的相关性研究

STUDY OF RELATIONSHIP BETWEEN CYCLIN DEPENDENT KINASE INHIBITOR p16 EXPRESSION AND DEVELOPMENT OF NEUROBLASTOMA

目的 :探讨细胞周期素依赖激酶抑制因子 p1 6表达与神经母细胞瘤 ( NB)发生发展的关系。方法 :应用原位杂交和免疫组织化学技术检测 2 6例 NB标本中 p1 6m RNA和蛋白产物表达。结果 :2 6例 NB中 ,应用原位杂交检测 p1 6m RNA表达未见阳性染色细胞 ;免疫组织化学技术检测发现 3例标本有 p1 6蛋白表达阳性细胞。结论 :神经母细胞瘤中无 p1 6表达或表达很低 ,其发生发展可能与细胞周期素依赖激酶抑制因子p1 6不表达或低表达、不能抑制细胞周期素依赖激酶促进细胞周期演进的功能关系密切。

Objective:To study relationship between the cyclin dependent kinase(CDK) inhibitor p16 expression and development of neuroblastoma(NB).Methods:26 specimen were examined by in situ hybridization technique and immunohistochemistry stain.Results:Positive cell of p16 expression was not detected in 26 cases by in situ hybridization,and positive cell of p16 expression was found in 3 cases by immunohistochemistry stain.Conclusion:The development of NB may be associated with no expression and low expression of CDK inhibitor p16.