超临界流体萃取法用于荜茇中胡椒碱定量测定研究

Determine Piperine in Fructus Piperis Longi by Supercritical Fluid Extraction

建立超临界流体萃取（ＳＦＥ）荜茇药材中的胡椒碱的方法。采用正交试验法优选ＳＦＥ 提取工艺，并用反相高效液相色谱法进行测定。在ＳＦＥ 中，确定萃取压力为３８．５ Ｍ Ｐａ，温度７０℃，改性剂量０．４ ｍ ｌ，静态萃取时间５ ｍ ｉｎ 及动态萃取体积５ｍ ｌ。荜茇药材中胡椒碱的平均回收率为９７．６８％ （ ＲＳＤ ０．８％ ）

To develop a supercritical fluid extraction (SFE) method for piperine in Fructus Piperis Longi. SFE technics was selected by orthogonal design, and HPLC was developed for the determination. SFE conditions: pressure 38.5 MPa, tempreture 70℃, 0.4 ml Meth as modifier, static extraction time 5 min and dynamic extraction volume 5 ml. The mean recovery of 97.68% ( RSD 0.8%) for piperine was obtained.