不同地区高良姜挥发油和1,8-桉油素含量测定比较

Determination of Volatile Oil and 1,8-cineole in Alpinia officinarum Hance from Different Areas by GC

按１９９５年版《中国药典》方法测定不同地区高良姜的挥发油含量，以及用环己酮作内标、程序升温毛细管气相色谱法测定挥发油中１，８－桉油素的含量。结果显示：不同地区高良姜的挥发油含量各有差异，在０．８４％～１．３０％之间，其主要成分１，８－桉油素含量为０．２６８％～０．５０１％，占挥发油的２９．８％～３９．８％。１，８－桉油素在２．７０９～６．５７４μｇ的范围内呈线性关系，γ＝０．９９９９，平均回收率为９９．３％（ｎ＝４，ＲＳＤ＝２．４％）。方法精密度高，重复性好，操作简便。

The content of volatile oil and 1,8-cineole in Alpinia officinarum Hance from different areas were determined by the methods in the Chinese Pharmacopoeia(1995)and capillary chromatography analysis with cyclohexanone as internal standard respectively.The result:the standard curve of 1,8-cineole was linear in the range of 2.709～6.754 μg, γ=0.9999,average recovery is 99.3%( n=4, RSD=2.4%),the contents of 1,8-cineole and volatile oil are 0.268%～0.501% and 0.84%～1.30% respectively.The method is simple and reliable,and its result is accurate and reproducible.