细胞因子和低亲合力IgE受体水平的变化与外源性哮喘的关系

The relationship between asthma and the changes in cytokins and low-affinity IgE receptor

目的 探讨T,S细胞异常活化在外源性哮喘免疫调节机制中的作用。方法 对30例外源性哮喘患者发作期和缓解期及30例健康成年人的外周血,采用ELISA双抗体夹心法,测定血清可溶性白介素-2受体(Soluble interleukin-2 receptor,sIL-2R)和白介素-4(Interleukin-4,IL-4)水平,用抗人CD_(23)的McAb测定CD_(23)。结果 外源性哮喘发作期血清sIL-2R,IL-4和CD_(23)水平明显升高,与缓解期和对照组有明显差异(P均<0.01),且CD_(23)水平分别与血清sIL-2R和IL-4之间有明显正相关(r=0.85,0.64,P均<0.01)。结论 T,B细胞异常活化是外源性哮喘发病的关键,通过测定细胞因子水平的变化,监测T,B细胞的活化,为今后临床干预哮喘的发生和治疗提供理论基础。

Objective To study the role of abnormal activation of T-cell and B-cell in the regulatory mechanisms is responsible for exogenous asthma. Methods The serum soluble interleukin-2 receptor (sIL-2) and interleukin-4 (IL-4 ) levels with ELISA and CD'23 with anti-human CD23 McAb were measured in peripheral blood of 30 cases of exogenous asthma at the attack and remission stages, and 30 healthy adults. Results Serum sIL-2R, IL-4 and CD23 significantly increased in cases of exogenous asthma at the attack stage as compared to those at the remission stage and healthy adults(F<0. 01). Also, CD23 level positively correlated with serum sIL-2R and IL-4 Level (r = 0. 85, 0. 64, respectively ,P<0.01). Conclusion Abnormal activation of T-cell and B-cell played an important role in the pathogene-sis of exogenous asthma. Measuring the changes in serum cytokins and abnormal activation of T-cell and B-cell provided a theoretical basis for the clinical intervention on its pathogenesis and management.