脑星形细胞瘤P^(53)抗癌基因突变的研究

Study of P^(53) tumor suppressor gene mutations in brain astrocytomas

目的研究Ｐ５３抗癌基因突变与脑星形细胞瘤恶性程度的关系。方法采用非同位素ＰＣＲ－ＳＳＣＰ分析法检测了３１例星形细胞瘤患者Ｐ５３抗癌基因的突变情况。结果（１）９例存在突变，突变区域分布于第５～８外显子中，以第６外显子突变率最高，第９外显子未测得突变病例。（２）２例存在多点突变，其中１例同时存在第５、６外显子突变，另１例同时存在第６、７外显子突变。（３）病理级别为Ⅰ、Ⅱ级组的突变率为０，Ⅲ级组的突变率为３６．４％，Ⅳ级组的突变率为５０％。Ⅲ、Ⅳ级组与Ⅰ、Ⅱ级组的突变率比较，均存在极显著差异（Ｐ＜０．０１）。

Objective To study the relationship between P^(53) tumor suppressor gene mutations and the malignant degree of brain astrocytomas.Methods Using nonisotope PCRSSCP molecular biological techniques,we detected the P^(53) tumor suppressor gene mutations in 31 specimens of astrocytomas.Results (1)Of all the 31 cases,P^(53) tumor suppressor gene mutations locating in exon 5～8 were detected,mutations were detected in 9 cases.Mutation rate in exon 6 was the highest,with no mutation being detected in exon 9.(2)Multiple point mutations were detected in 2 cases,one in exon 5～6 and the other in exon 6～7.(3)Mutation rate of pathological grade Ⅰ～Ⅱ group was O,that of grade Ⅲ group and grade Ⅳ group were 36.4% and 50%,respectively.The discrepancy of mutation rate between gradeⅠ～Ⅱ group and grade Ⅲ～Ⅳ group were significant (P<0.01).Conclusion The investigation demonstrated that P^(53) tumor suppressor gene mutation had a close relation to the malignant degree of astrocytomas.