Svate-3与NGF、GM1对家兔脊髓缺血损伤的作用

Effects of Svate-3, NGF and CM1 on Ischemic Spinal Cord Injury in the Rabbits

本实验旨在证实３种药物对神经损伤的作用及用药方法．方法：实验选用日本大耳兔２４只，分５级：Ａ组－正常组（致伤前）；治疗组：Ｂ组──Ｓｖａｔｅ－３＋ＮＧＦ＋ＧＭ１；Ｃ组─ＮＧＦ＋ＧＭ１；Ｄ组－Ｓｖａｔｅ－３；Ｅ组─生理盐水（ＮＳ）对照组．采用清醒家兔肾动脉下腹主动脉（ＩＲＡ）阻断血流，造成脊髓１２以下节段缺血模型，缺血时间为２０ｍｉｎ，观察２４小时，测定脊髓诱发电位（ＭＥＰ，ＳＥＰ），脊髓血流量（ＳＣＢＦ），血液粘度和血气．结果：ＭＥＰ潜伏期伤后治疗组和对照组均比伤前延长。Ｐ＜０．０５．治疗Ｂ组和Ｃ组在伤后８小时和２４小时潜伏期延长Ｄ组和对照组在伤后４小时、６小时、８小时和２４小时均有显著延长（Ｐ＜０．０１）．ＳＥＰ潜伏期Ｂ、ＣＤ、Ｅ组均比损伤前Ａ组延长．Ｃ组在４小时，２４小时潜伏期缩短．Ｂ、Ｃ组优于其它组．ＳＣＢＦ：Ｂ、Ｃ、Ｄ组与损伤前比较，没有显著差别（Ｐ＞０．０５）．损伤后１小时、２４小时ＳＣＢＦ下降．全血粘度（ＢＣＰ）和血浆粘度Ｂ组、Ｄ组在２４小时均下降，Ｐ＜０．０５．Ｅ、Ｃ组比伤前升高，Ｐ＞０．０５．血气ｐＨ均在正常范围，各组血气指标没有显著差别（Ｐ＞０．０５）结论：ＮＧＦ＋ＧＭ１或Ｓｖａｔｅ－３＋?

Objective: To investigate the function and using methods of three drugs (snake venomantithrombus enzyme (Svate-3), nervous growth factor (NGF) and monosialoganglioside (GM1) forischimc spinal cord injury. Methods: 24 Japan-big-eared rabbits were routinely divided into 5 groups. Ingroup A. the rabbits had no nerve injury. In group B, rabbits were treated with svate-3 only. Group E withnounal saline (NS) as the control group. The animal model of sibal cord ischemia in awake rabbits wasmade by occluding the nephro-hyparterial aorta. The ischemia time was 20 minutes. The spinalcord evoked potential (MEP and SEP). spinal cord blood flow (SCBF), blood viscosity and blood gas were measured in 24 hours . Results .MEP (motor evoked potential): The latent period in group E. C. D and E isloger fhan that in group A (p<0.05-0.01). It Prolonged the 8h and 24h hours in group B and C, and itprolonged sighficantiy at the 4, 6, 8 and 24 hours in group D and E(P<0.01). SEP (sensory evokedpotentia); The latent period in group B, C, D and E is loger than that in group A. It shortened at the 4 h and24 h in group B and C. Group B and C is superior to the other groups. SCEB (spinal cord blood flow): ingroup B, C and D, it declined at the 1 and 24 hours (p<0.01 ). Blood viscosity (BCP and PCP) in group B andD declined at the 6 and 24 hours (P<0.05), and it rose in group E and C compared with group A (P<0.05).Conclusion: Using svate-3, NGF and GM1 or using NGF and GMI is supesrior to using svate-3 only fornerve injury repair. The three drugs have no effect on blood gas.