乙烯释放剂克服香石竹试管苗玻璃化的研究

STUDIES ON THE PREVENTION OF VITRIFICATION OF IN VITRO CULTURE OF CARNATION BY USING ETHYLENE RELEASERS

用香石竹(Diamthus caryphyllus L.)为试验材料,乙烯前体ACC和乙烯释放剂乙烯利为生长调节剂进行试验。试验结果表明,ACC对克服香石竹试管苗玻璃化有显著效果,并能一次成苗,正常试管苗的诱导频率达98％。从外部形态观察,茎叶充实度好,根系发达;茎横切面显微观察,茎内厚壁组织发达,细胞排列紧密;生理指标测定结果,蛋白质、干物质、叶绿素含量均高于玻璃苗,干物质为玻璃苗的1.7倍,叶绿素为玻璃苗的5.5倍。乙烯利培养的试管苗生长情况与ACC培养的相近。添加ACC、乙烯利的培养瓶中,乙烯释放量明显高于对照,说明乙烯对克服香石竹试管苗玻璃化有良好作用。

Experiments were carried out with carnation by using ethylene releasers as growth regulators suppiemellted in the media. Results showed that ACC, the immediate precursor of ethylene, exhibited significant effect on preventing the vitrification of in vitro culture of carnation, and obtaining full - grown plantlets without subculture. The induction frequency of normal planllets was 98%. A compact stem and leaf and good root system was observd in in vitro culture. Under microscopical examination, closely arranged cells and well developed sclerenchyma tissue were found in cross sections of the stem. Biochemical analysis revealed that the dry matter and chlorophyll contents were higher than those of vitrified ones by 1.7 and 5. 5 times, respectively. The growth of plantlets induced by CEPA were similar to those by ACC. The quantity of ethylene released in the culture tubes where the media was supplemented with ACC or CEPA was found to be much higher than that in the control lubes, implying that ethylene had good effect on vitrification prevention.