水稻光温敏核不育基因与同工酶基因的遗传关系及连锁测定

STUDY ON THE GENETIC RELATIONSHIPS BETWEEN PHOTOPERIOD-TEMPERATURE SENSITIVE GENIC MALE STERILITY GENES AND ISOZYME GENES AND ITS LINKAGE MEASUREMENT IN RICE (Oryza sativa)

本试验以光温敏核不育系农垦58S,双8-2S,N_(989)为材料,以花粉育性为指标,研究了不同自然条件,不同光照长度及不同温度处理下同工酶变化与育性变化的相关性,并测定了同工酶基因与光温敏核不育基因的连锁关系。试验结果表明:Adh-1和Est-3与育性有密切关系,不育时,这两位点都表现有效等位基因;可育时,这两位点都表现无效等位基因。控制光温敏核不育的两对基因中,一对与Adh-1密连锁(重组率为0);另一对与Cat-1连锁(重组率为29％)。

In the present experiment, the correlations between the changes of isozymes and fertility under different natural conditions, different day-light lengths and different temperature treatments were studied by means of the pollen fertility index, using the photoperiod-temperature sensitive genie male-sterile lines (NongKen58S, Shuang8-2S, N9RS). At the same time, the linkage relationships between isozyme genes and sterility genes were tested. The results indicated that there were close relationships between Adh-1, Est-3 and sterility. In the case of fertility and sterility, both of the loci expressed null alleles and active alleles respectiv -ely. One of sterility genes Jinked to Adh-1 with a recombination value of 0, another gene linked to Cat-1 with a recombination value of 29 percent.