官井洋野生与养殖大黄鱼同工酶的研究

ISOENZYMES Of WILD AND REARED Pseudosciaena crocea

应用聚丙烯酰胺凝胶电泳(PAGE)法对取自福建宁德官井洋海区养殖群体和野生种群各20尾大黄鱼的9种同工酶15个基因座位进行检测分析。结果表明,大黄鱼眼球中乳酸脱氢酶(LDH)酶谱表现为LDH的经典模式即有5种谱型的LDH。在所检测出的15个基因座位中 ,野生种群样品的编码苹果酸脱氢酶(sMDH)、苹果酸酶(ME)和琥珀酸脱氢酶(IDDH 1)的3个基因座位具有多态性 ;养殖群体中仅发现1个基因座位具有多态性(IDDH 1)。所检测的野生种群比养殖群体的遗传变异水平高。但从总体上来说 ,官井洋大黄鱼遗传多样性相对较低 ,表明该种群已出现了种质退化现象。因此 ,进行科学的遗传管理对保护和恢复大黄鱼资源是相当必要的。

Genetic diversity of 9 isoenzymes located in both reared and wild large yellow croaker Pseudosciaena crocea (Richardson) collected from Guanjingyang in Ningde, Fujian on May 29, 1999 was investigated by polyacrylamid gel electrophoresis (PAGE) in this paper. The genetic diversity of wild population was relatively low, with 20% of the polymorphism and 0.017 of the mean heterozygosity, while three polymorphic loci were detected. But the genetic diversity of reared stock was even lower than that of the wild one with just a polymorphic locus found. These results showed the good genetic characters of this fish has greatly been lost in both stocks distributed in the coastal waters of Fujian and eastern Guangdong. So the appropriate management should be conducted in rearing P.crocea industry.