维生素E对体外培养的人眼小梁细胞糖胺多糖代谢的影响

Effect of Vit E on the glycosaminoglycans metabolism of cultured human trabecular meshwork cells in vitro

目的探讨维生素Ｅ对体外培养的人眼小梁细胞糖胺多糖（ｇｌｙｃｏｓａｍｉｎｏｇｌｙｃａｎ，ＧＡＧ）代谢的影响。方法第３～５代人眼小梁中加入１００、２００、４００、８００μｇ／ｍｌ的ＶｉｔＥ，分离提纯后ＧＡＧ经腊酸纤维薄膜电泳、闪烁测量测得人眼小梁细胞ＧＡＧ的总量和各组份构成比。结果１００、２００、４００μｇ／ｍｌＶｉｔＥ能增加ＧＡＧ的总量，同时增加透明质酸的构成比，降低含硫ＧＡＧ的构成比。８００μｇ／ｍｌＶｉｔＥ降低ＧＡＧ总量而不改变其构成比。结论ＶｉｔＥ似有在发病学上防治ＰＯＡＧ的可能性。

ObjectiveTo study the influence of Vit E on glycosaminoglycans (GAG) metabolism of cultured human trabecular meshwork cells.MethodsVit E in different concentrations of 100,200,400 and 800 μg/ml medium were added to the cells to the third to fifth generation.After extracting and purification,an aliquot of GAGs was used for cellulose electrophoresis and scintillation counting to determine the total amounts and their composition.Results100,200,400 μg/ml Vit E increased the total GAGs in the human trabecular meshwork cells.They also increased hyaluronic acid and decreased sulfatedGAGs.800 μg/ml Vit E descreased the total GAGs but keep the composition.ConclusionVit E can influence trabecular meshwork cells GAG metabolism.It is suggested that Vit E may be utilized to reverse the pathogenesis of POAG.