巨噬细胞诱发的实验性PVR玻璃体中的IL-6

Interleukin6 in experimental proliferative vitreoretinopathy induced by macrophages

目的检测增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）发生过程中玻璃体内白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）的含量变化，探讨其在ＰＶＲ早期免疫反应中的作用。方法用同种巨噬细胞诱发兔眼ＰＶＲ，在不同时间点抽取玻璃体液，采用双抗体夹心法ＥＬＩＳＡ试剂盒检测ＩＬ－６含量。结果ＩＬ－６含量１４ｄ达高峰９９８ｐｇ／ｍｌ，２８ｄ降至其基线水平；拟合二次曲线提示玻璃体中ＩＬ－６含量在１０～２１ｄ呈一高水平平台２５１ｐｇ／ｍｌ。结论ＩＬ－６在此ＰＶＲ模型中玻璃体含量在第２周明显升高，并持续至第３周，与ＰＶＲ形成中的第二阶段细胞增殖期相一致，提示ＩＬ－６对此ＰＶＲ模型细胞增殖、特别是对免疫活性细胞具有调控作用。

ObjectiveTo measure the changes in concentration of interleukin6 (IL6) during the development of experimental proliferative vitreoretinopathy(PVR) induced by macrophages and explore to role in the early stage of the immune reaction of PVR.MethodsRabbit PVR model was induced by injection of homogenous macrophages.The vitreous,aqueous and peripheral venous blood were taken at different periods and IL6 levels in them were measured with an ELISA assay.ResultsThe IL6 levels reached a peak (998 pg/ml) on d 14 in the model ,showing its high plateau of 251 pg/ml from 10 to 21 d,then decreasing to 0 pg/ml at d 28 after macrophage injection.ConclusionThe changes in IL6 concentration in the model correlated with the cellular proliferation during the early stage of PVR,revealing that IL6 may modulate cellular proliferation,especially immunologic cells.