摘要
以产生a-淀粉酶的解淀粉芽孢杆菌(Bacillusamyloliquefaciens)WD-21和不能利用淀粉的产生肌苷的枯草芽孢杆菌(BaclllusSubtills)HW-9(ade,NP-,8-AG')为亲株,在分别以0.75和0.5mg/ml的溶菌酶浓度作用下形成原生质体,在电镜观察下,其形成率为99%。通过优化融合条件,即35℃在PEG-1000溶液中融合,融合率达到3.11×10-6。得到的融合子在选择培养基上传代多次,淘汰掉不稳定的融合子,得到稳定的融合子F16和F129具有产生a-淀粉酶的能力,在以淀粉为碳源的发酵培养基中摇瓶发酵试验,F16菌株产生肌苷的能力接近6g/L.
wo parent strains,
Bacillus amyloliquefaciens WD-21 with erythromycin resistance producing a-amy-lase and
Bacillus subtilis with 8-AGr resistance producing inosine, are fused by polyethylence
glycoltreatment to form protoplast.The optimum condition for ptotoplast formation, regeneration
and fusionhave been studied.The frequency of fusion is 3.11×10-6.The stable fusants are
selected by 10 mg/L Ery and 100 mg/L 8-AGr medium plates. The fusants F16 obtained can
preduce inosine in fermenta-tion broth in which starch is carbon source,the concentration of
inosine is 5.89 g/L.
出处
《药物生物技术》
CAS
CSCD
1995年第2期7-10,共4页
Pharmaceutical Biotechnology
基金
江苏自然科学基金
关键词
枯草芽孢杆菌
解淀粉芽孢杆菌
原生质体融合
肌苷
发酵
Bacillus subtilis
Bacillus amyloliquefaciens
Protoplast fusion
lnosine fermentation