摘要
以４％琼脂糖为载体，对甲苯磺酰氯为活化剂，对胰蛋白酶的固定化条件及固定化酶的性质进行了研究。其固定化条件为ｐＨ７．４，温度１５℃，时间８ｈｒ，酶量２０ｍｇ／ｇ（ｗｅｔ－ｇｅｌ）。同时研究了温度、ｐＨ值、时间以及载体与酶的比例等因素对联蛋白酶固定化的影响。最后报道了该固定化酶的最适ｐＨ，热稳定性，ｐＨ贮存稳定性和操作稳定性。用这种固定化胰蛋白酶纯化抑肽酶，抑肽酶纯度提高５０倍，回收率在８５％～９５％。
his paper emphasized the study of immobilization of
trypsin and the properties of immobillzed trypsinunder the condition of sepharose-4B as
support and tosyl chloride as activating agent. vve found thatthe immobilizing condition was as
follows:pH value., temp reaction time and trypsin were 7.4,15℃,8h.
20mg/lg(wet-gel)respectively. The effects of temp.,pH value,immobilizing time and the ratioof
trypsin to sepharcoe-4B on trypsin immobilizition were studied. Trasylol was obtained from
bovine lung extract, after affinity chromatography on immobilitedtrypsin. Its purity was raised
50 times, the rate of recovery was 90%～95%. We reported some findings of immobilized
trypein:Optimum pH, heat steady, Ph store steady and oper-ation steady of immobilized trypsin
as well. Some parameters of immobilized trypein were also deter-mined.
出处
《药物生物技术》
CAS
CSCD
1995年第4期20-23,共4页
Pharmaceutical Biotechnology
