摘要
本文采用大肠杆菌核苷磷酸化酶，以５－氟尿嘧啶（ＦＵ）、２＇－脱氧尿苷（ｄＵ）和磷酸盐为底物酶法合成２＇－脱氧－５－氟尿苷，实验结果表明，含１０ｍｍｏｌ／ＬｄＵ、３０ｍｍｏｌ／ＬＦＵ、１０－３０ｍｍｏｌ／Ｌ磷酸盐的混合物加入５％培养２４ｈ的大肠杆菌湿菌体于ｐＨ７．０反应３ｈ，底物ｄＵ的转化率可达４５．９％。
he enzymatic synthesis of2'-deoxy-5-fluoro-urluine with E. coli
nucleoside phosphorylase was stud-ied. The substrates of it were
5-fluorouracil,2'-deoxy-uridine and potassium phosphate. The experi-ment showed that the
optimum substrates concentration was 10 mmol/L dU , 30 mmol/L FU,10-30mmol/L potassium
phosphate. When we added 5%(w/V) E. coli wet cell cultured for 24h to the re-acting mixture
and adjusted pH to 7.0 with KOH, the biosynthesis took place at 70℃ for 3 h effective-ly and
45.9%dU was converted to FdU.
出处
《药物生物技术》
CAS
CSCD
1995年第4期24-27,共4页
Pharmaceutical Biotechnology
