丙型肝炎病毒感染与HCC相关性的研究

Study on the relativity of hepatitis C virus infection and hepatocellular carcinoma

目的 探讨丙型肝炎病毒 (HCV)感染与肝细胞癌发生的关系。方法 用聚合酶链反应 (PCR)对淮南地区 92例肝癌、117例肝硬化和 10 0例肝癌切除的肝组织标本进行HCV RNA检测 ,并以限制性片段长度多态性分析对其中 89例HCV RNA阳性血清进行了HCV基因分型。结果 肝癌组HCV感染率 2 9 3% ,肝硬化组HCV感染率 2 6 5 % ,肝组织标本组HCV感染率 31 0 % ;各组均以HCVⅡ型为主(6 5 2 % ) ,HCVⅢ型次之 (31 5 % )。结论 淮南地区肝癌的诱发与HCV感染密切相关 ,HCVⅡ型在本地区HCV相关性肝癌和肝硬化的发生中可能起主要作用。

Objective To study the relationship between HCV infection and hepatocellular carcinoma. Methods PCR techniques were used to detect the serologic markers of HCV-RNA of 92 patients with hepatocellular carcinoma (HCC), 117 patients with liver cirrhosis (LC) and 100 paraffin-embedded liver tissues from patients from Huainan area. The 89 HCV-RNA positive sera from the 209 patients were analyzed by restriction fragment length polymorphism (RFLP). Results The infection rates of HCC, LC, liver tissues were 29.3%, 26.5%, 31.0%. HCV genotype of all groups was mainly of type Ⅱ (65.2%) whereas that of type Ⅲ was 31.5%. Conclusion HCC is associated with the infection of HCV in Huainan area. The infection of HCV type Ⅱ appears to play a main role in the development of HCV-associated HCC and LC in this area.