GA_3和PP333对黄化黄瓜子叶中NPR蛋白Pchlide和Chla含量的影响

THE EFFECT OF GA_3 AND PP333 ON THE CONTENT OF NPR PROTEIN PCHLIDE AND CHLA IN ETIOLATED CUCUMBER COTYLEDONS

1.GA_3外理可使Pchlide含量增加,且在一定范围内其含量与GA_3浓度呈正相关.同时,GA_3可减缓光下Pchlide骤减的速度,并使其在一定时间内维持较高的水平,GA_3亦使Chla含量提高,并具有缩短叶绿素a合成停滞期的作用.免疫分析表明,GA_3处理后NPR蛋白含量略高于对照.2.PP333处理后其效果与GA_3相反,Pchlide、Chla和NPR含量均低于对照,并使Chla合成停滞期延长.

1 . The content of Pchlide in etiolated cucumber cotyledons increased after the cotyledons were treated with GA3. Rise in the concentration of GA3 gave rise to the content of Pchlide. Pchlide in etiolated cucumber cotyledons decreased readily under light, and GA3 could delay this procss, which maintained a higher level of Pchlide in the time period. When etiolated cucumber cotyledons were treated with GA3, the content of Chla increased and the lage phase of Chla synthesis was shorter than that of the control. According to the immunoblot assay, the content of NPR protein also increased. 2 . When etiolated cucumber cotyledons were treated with PP333, the content of Pchlide, Chla and NPR protein decreased, and the lag phase of Chla synthesis was longer than that of the control.