摘要
目的 检测大鼠牙槽骨成骨细胞中IL-1β在正常及增强咀嚼压力状态下的动态表达,探讨IL-1β在牙槽骨改建中 的分子机制。方法采用HE染色和免疫组化的方法,观察牙周形态变化以及牙槽骨成骨细胞中IL-1β蛋白表达。结果 生理限度内咀嚼压力增强时,形态学显示大鼠牙周膜增宽、牙槽骨新骨形成;免疫组化观察到成骨细胞中IL-1β中表达 较正常咀嚼压力时明显增强。结论咀嚼压力增强促使牙周组织产生IL-1β明显增多,诱发了破骨功能,同时,还激活了 成骨功能。提示IL-1β在咀嚼压力影响牙槽骨改建的过程中起着重要的调节作用。
Objective To explore the molecular mechanism of alveolar bone remodeling by studying the dynamic changes of IL-1β expression in rat alveolar bone osteoblasts. Methods Rat models of increased bite force of the back teeth were established, and the expression of IL-1β in the alveolar bone osteoblasts were determined by HE staining and immunohistochemistry. Observation of the changes in the histological morphology of the periodontium was conducted microscopically. Rats with normal bite force served as control. Results The increase of bite force (within the physiological limit) induced the widening of the periodontal ligament and the osteogenesis in the alveolar bone. Significant enhancement of IL-1β expression was observed in the osteoblasts of rats with increased bite force, in comparison with that in the rats with normal bite force. Conclusion Increased bite force causes higher expression levels of IL-1β in the alveolar bone osteoblasts, initiating the destruction process of the bone but simultaneously the activation of the ossification, suggesting that IL-1β plays an important role in the regulation of periodontium remodeling in response to changes in the bite force.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第4期276-278,F004,共4页
Journal of First Military Medical University
关键词
咀嚼压力
白细胞介素-1Β
牙槽骨
改建
免疫组织化学
大鼠
bite force
interleukin-1β
alveolar bone remodeling
immunohistochemistry 中图分类号:R33-33 文献标识码:A