K562细胞7种珠蛋白基因mRNA水平的研究

Comparative study of the mRNA levels of 7 globins in K562 cells

目的研究Ｋ５６２细胞ａ、β、Ｇγ、Ａγ、ζ、ε、δ、珠蛋白基因ｍＲＮＡ水平。方法采用ＲＴ－ＰＣＲ在相同条件下同时扩增成 功７种珠蛋白ｍＲＮＡ，比较Ｋ５６２细胞内各珠蛋白ｍＲＮＡ水平。结果α基因簇中α－ｍＲＮＡ水平明显高于ζ－ｍＲＮＡ，β 基因簇ｍＲＮＡ表达水平依次为Ｇγ、Ａγ、ε、δ，Ｋ５６２细胞不表达β－珠蛋白ｍＲＮＡ。结论转录后水平的调控可能在ｋ５６２ 细胞α基因簇的表达中起重要作用，β基因簇各珠蛋白ｍＲＮＡ水平和蛋白质水平基本相符。

Objective: To investigate theα、β、Ｇγ、Ａγ、ζ、ε、δ、globin mRNA levels in K562 cells. Methods The technique of RT-PCR was applied successfully to amplify 7 globin mRNA in K562 cells, And comparative study of the relative amount of the 7 globin mRNA was performed. Result The expression level of α-mRNA in a globin gene cluster was higher than that of ζ-mRNA. The order of mRNA levels in β globin gene cluster was . β mRNA was not detected in K562 cells. Conclusion The expression level of mRNA in β globin gene cluster is consistent with that of β-like globin, the post transcriptional up- and down-regulation might be crutial in globin gene expression.