口炎冲剂抗Ⅰ型单纯疱疹病毒机制的实验研究

Experiment Study on the Role of Anti—Herpes Simplex Virus I of Kou Yan Granules

用细胞致病效应微量抑制法和人成纤维细胞干扰素体外诱生法探讨口炎冲剂抗单纯疱疹病毒Ⅰ型的作用机制。结果表明,口炎冲剂具有抗 HSV—Ⅰ_(2128)株的能力,是间接的,并只影响病毒繁殖的转录水平,可能是通过诱导Ⅰ型干扰素的产生实现的,在 HEL 细胞上诱生出的干扰素效价为13.26lu/ml。

Kou Yan Granules(KYG)is consisted of eleven Chinese medical herbs.The presentstudy was carried out by using microdosage cytopathic effect(CPE)method and β-interferon-inducing effect in vivo experiment The result showed that KYG has anti-HSV-I effect,can indirectly restrain the replication HSV-I 2128 strain but not spoilthe DNA and mRNA of the virus.KYG may be a inducer of type I-I FN,because KYGcan induce human embryo lung fibroblast cell(HEL)producing I-I FN,the interferontiter being 13.26 IU/ml.The result suggested that the capacity of anti-HSV-I ofKYG is achieved by inducing interferon.