五氧化二钒对大鼠胚胎肢芽细胞增殖和分化的影响(英文)

Effects of V2O5 on Proliferation and Differentiation of Limb Bud Cells of Rat

五氧化二钒（Ｖ２Ｏ５）是一种常见的工业毒物和环境污染物。为了进一步验证钒的致畸性，并为揭示其发育毒性的作用机理提供依据，本文在大鼠致畸试验的基础上，应用细胞微团培养方法观察了钒对大鼠胚胎肢芽细胞在体内外增殖和分化的影响。体外实验：从妊娠ｄ１３的孕鼠中分离出胚胎，取前肢芽，制成细胞悬液（２ｘ１０７ｃｅｌ／ｍＬ），取１０μＬ置于培养板上成一微团，于高湿、３７℃的ＣＯ２孵箱中预培养２ｈ，然后缓慢加入不同剂量的Ｖ２Ｏ５溶液（勿冲散微团，对照加培养基），培养５ｄ。体内实验：取鼠妊娠ｄ１２时，经ｉ．ｐ，以不同剂量的Ｖ２Ｏ５处理，１６ｈ后处死、取胚胎肢芽细胞培养。然后，固定细胞，分别采用苏木精染色和阿利新兰染色，前者经甲醇脱色后以酶标光密度值反映细胞增殖情况；后者由镜检每一微团中天蓝色的分化集落数判断对细胞分化的抑制作用。体外试验结果显示，钒剂量在５．０μｍｏｌ／Ｌ以上时细胞集落数明显下降，光密度则在７．５μｍｏｌ／Ｌ以上时明显下降，均呈明显的剂量反应关系（Ｔａｂｌｅ１），说明钒对肢芽细胞增殖和分化都有抑制作用，但细胞增殖和分化的两条抑制曲线明显不同（Ｆｉｇ．１），其ＩＣ５０分别为１３．１４和４．７７μｍｏｌ／Ｌ。按Ｆ?

The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells. 