摘要
The Sox genes of P. dabryanus were amplified by degenerate primers within HMG-box. From male and female individuals, three amplification bands with the lengths 220, 550 and 1500 hp were displayed, respectively. A fragment of potential novel gene was displayed in the band of 550 hp. It possibly coded 53 amino acid residues, whose amino acids had sequences in 96%, 94%, 90% and 47% similarities to those of the genes Sox 8, Sox 9, Sod o and SRY of mouse, and in 64%, 94%, 58% and 40% similarities to them of human, respectively. Accordingly, it was named PdSox 8. Northern blotting result indicated overexpression of it in adult liver and brain.
鱼类在脊椎动物系统进化过程中起着承先启后的作用，其性别决定具有原始性、多样性和可塑性。深入研究鱼类的性别决定和分化具有重大的理论意义。Sox基因家族是九十年代发现的一个新的基因家族,其中的许多成员都与性别决定和分化具有直接的关系。大鳞副泥鳅是一种常见的小型鱼类，属于鲤形目鳅科，是少数具有异形性染色体中的一种。本文根据已发表的Sox蛋白质的序列资料，选择在不同物种中保守程度最高的区段设计兼板，在扩增产物中有三条主带，其大小分别为220bp550 bp和1500bp，另有一条相当弱的带，大小为700bp（Fig.1)。雌雄个体中扩增结果一致。经克隆和DNA序列分析，从550bp扩增带中得到一新的基因片段，长500bp，编码53个氨基酸；其余部分长340bp，可能为一内含子，且符合“GT…AG”规律（Fig．2)。其可能编码的蛋白质氨基酸序列与小鼠的Sox8，9，10，SRY基因的相似性分别为96％，94％，90％和47％；与人类SOX8，9，10，SRY基因的相似性分别为64％，94％，58％和40％（Fig.3)。根据这些特性将命名为PdSox8。Northern杂交结果表明PdSox8在成体脑及肝脏组织中有宏量表达（Fig.4）。
基金
his project was supported by the National Doctorate Research Foundation of China
the National Natural Science Foundation of
