摘要
DNA expression constructs containing hGH gene directed by bovine αsl-casein gene regulatory sequences were injected into mouse heart or mammary glands in vivo. hGH expression was readily detected by radioimmunoassay in milk of the nursing mice throughout lactation. Our results demonstrated the value of this simple and rapid approach in screening gene constructs and identifying transcription regulation sequences in vivo prior to the generation of transgenic animals. For the First time we report the successful expression in mammary gland via intracardial injection.
以牛ａｓｌ－酪蛋白基因的５’端及上游区３．４ｋｂ和３’端及下游区３．５ｋｂ作为调控元件，以人生长激素基因作为报告基因构建了融合基因，通过乳腺、心脏注射到小鼠体内，利用放射免疫分析方法（ＲＩＡ）在泌乳期小鼠的乳汁中检测到了表达的人生长激素，表明融合基因的构建是正确的。并由此建立了通过靶组织注射结合心脏注射对融合基因的构建进行考察的快速简便方法。其中通过心脏注射而于乳腺获得特异性表达的实验结果尚未见报道。
