615小鼠血红蛋白珠蛋白α链的分离纯化及氨基酸组成

The Purification and Amino Acid Composition of α Chain of Hemoglobin of 615 Mice

用CM cellulose 2 3柱层析分离纯化了 61 5小鼠珠蛋白α链 ,测定其N 末端氨基酸为缬氨酸。 61 5小鼠珠蛋白α链含有 1 4 1个氨基酸残基 ,其中含 1 9个亮氨酸残基 ,1 0个组氨酸残基 ,9个缬氨酸残基 ,上述三种氨基酸残基的数目与文献中亲本不同。

The α chain of hemoglobin of 615 mice was isolated and purified by CM cellulose 23 column chromatography method. The N terminal amino acid of α chain was determined with DABITC/PITC method and it was valine. The amino acid composition was determined and it was different from the parent's(C57BL) in the literature on the number of leucine residue, Histine residue and Valine residue.