食管癌及癌旁组织中人乳头状瘤病毒DNA的存在状态

The Status of Human Papillomavirus 16 DNA in the Tissues of Human Esophagus Carcinoma

作者以人乳头状瘤病毒(HPV)16DNA为探针,采用斑点杂交及Southern分子杂交技术检测24例食管癌及其相应癌旁组织中的HPV16DNA。结果:在食管癌组织中,HPV 16DNA斑点及Southern杂交的阳性率为50％(12/24),癌旁组织为37％(9/24);经Bam H I酶切的食管癌及癌旁组织中,杂交后出现7.2kb的HPV16DNA特异性阳性区带;经Pst I酶切杂交后出现2.7kb、2.3kb、1.75kb、1.18kb四条HPV16DNA阳性区带;未经酶切者来发现阳性杂交带出现。提示人乳头状瘤病毒基因组确实存在于食管癌及癌旁组织DNA中,且多以整合状态存在。

DNAs from 24 cases ofhuman esophagus carcinoma and 24 casesof corresponding para-carcinoma tissueswere datected by dot hybridization andSouthern hybridization techniques usinghuman papillomaviruis(HPV) 16 type DNAas probe. The results indicated that thepositive rate of the HPV 16 DNA Hybridi-zation was 50% (12/24) in esophagus car-cinoma tissues, and 37% (9/24) in para-carcinoma tissues. The resrlts of Southernhybridization showed obviously the posi-tive hybridizing band in the zone of 7.2kbin smear. It implied that HPV 16 DNAwas possibly integrated into the tissue′sDNA of esophagus carcinoma. In the fetalesophagus tissues as negative control, 3cases out of 4 showed positive results. Thesignificance has to be further investigated.This experimed has studied the status ofHPV 16 type in the issues of esophaguscarcinoma and para-carcinoma at molecularlevel. This is just a study of the virusetiology of esophagus carcinoma.