温石棉与煤尘致叙利亚地鼠胚胎细胞体外转化的实验研究

Experimental Study of Syrian Hamster Embryo Cell Transformation Induced by Chrysotile Fibers and Coal Dusts in Vitro

作者用SHE细胞体外转化试验,检测了新康温石棉和嘉阳煤尘的致癌性,并以甲基胆蒽作阳性对照,单纯培养液为阴性对照。结果显示:阴性对照组无自发性转化,阳性对照组和石棉的两个剂量组均出现细胞转化,煤尘组未出现转化现象。各转化灶阳性组与阴性对照组间均有显著性差异(P<0.05)。上述表明:四川新康温石棉具有致癌潜能,而嘉阳煤尘不能致SHE细胞发生转化。

We studied the Syrian ham-ster embryo (SHE) cell transformation byfocus assay for detecting the carcinogene-city of chrysotile asbestos fibers collectedfrom Sichuan Xinkang Asbestos Mine andparticulate coal dusts from Jiayang CoalMine. Both these mines belong to SichuanProvince. Results showed that transformedfoci were found. in the methylcholanthrane group and in the two asbestos dosinggroups(8μg/ml and 4μg/ml), whereas notransformed foci were noted in the coaldust groups and no spontaneous transfor-mation in the negative control groups.Morphologically,the transformed foci weredocumented by light and electronmicro-scopy. Signi ficant differences betweenthe positive transformed groups and thenegative control groups were demonstratedby statistic analysis. The results indicatethat Sichuan Xinkang chrysotile asbestosfibers are of carcinogenic potentiality,and Jiayang coal dusts do not inducetransformation in SHE cells.