纤维素分解菌对提高大豆蛋白溶出率作用研究

Study on the lncreasing Effect of Active Cellulase Producing Strains to the Rate of Solvable Soy Proteins

利用纤维素酶分解菌的固态发酵法降解高变性脱脂豆粕中的纤维素，研究其对提高豆粕中蛋白质水溶出率的作用。通过菌种筛选，确定了固态发酵用菌，分别是康氏木霉 13006和康氏木霉 13036，两株菌固态发酵条件为：料水比 =2∶ 3、 pH=4. 5、 28℃培养 4d后升温至 50℃培养 3d。在上述条件下，降解高变性豆粕中纤维素，水解后豆粕中蛋白质水溶出率分别达到 29.05％、 27. 06％。最后，利用蛋白酶来水解纤维素被降解后的豆粕，蛋白质水溶出率达到 76.21％、 75. 80％、 73. 52％，比仅以蛋白酶水解豆粕的蛋白质水溶出率 (67.87％ )高，说明通过降解纤维可显著提高高变性脱脂豆粕中蛋白质的溶出率。

The solid state fermentation with active cellulase producing strains in increasing the rate of protein dissolved of the high denatured defatted soybean cake was discussed in this thesis.By experiment,we select Trichoderma.Koningii 13006 and 13036 as the strains used in solid state fermentation And the optimum conditions of solid state fermentation is raw Material∶ watel=2∶ 3,pH=4.5,stay at 28℃ till 4 days,then improved to 50℃ ,3days.Thus,by the degradation of cellulose,the rate of solvable protein reached to 29.05％、 27. 06％ .At last,by using proteinase to hydrolyzing soybean cake of which the cellulose was degraded,the rale of protein dissolved was improved from 67.87％ to 75.80％、 73. 52％ .