肝脏同工铁蛋白性质的研究

A Study on Characters of Liver Isoferritin

本文研究了Wistar大鼠、正常人及肝细胞肝癌(HCC)病人的肝脏同工铁蛋白的性质。HCC病人的肝脏同工铁蛋白的铁与蛋白质比值为0.021,约为正常人的1/12。正常人及HCC病人的肝脏同工铁蛋白的分子量分别为20.5及21.5kd。鼠肝及HCC病人的肝脏同工铁蛋白的PAGE呈现一条区带,正常人肝为两条区带。正常人及Wistar大鼠肝脏及马脾同工铁蛋白的双向免疫扩散存在交叉反应。电镜观察到HCC病人肝脏同工铁蛋白颗粒疏松,大小不均。

The characters of liver iso-ferritin from Wistar rat, normal humanand patient with hepatocellular carcino-ma (HCC) were studied. The ratio of ironto protein of liver isoferritin from pa-tient with HCC were 0.021, which accou-nts for about one-twelfth of that of liverisoferritin from normal human. The mol-ecular weights of liver isoferritin fromnormal human and the patient with HCCwere 20.5 kd and 21.5 kd, respectively.The electrophoresis patterns of liver iso-ferritin from both Wistar rat and patientwith HCC showed one band,but two bandsfrom normal human. There was crossreaction in immunity among isoferritinsfrom normal human liver, Wistar ratliver and horse spleen by double immuno-diffusion. The PAGE patterns of liver iso-ferritin from different sources could beclearly differentiated. The data presentedmay serve as a good reference for furtherstudy of the relationship between thestructure and function of ferritin and maybe used practically in clinical medicine.