国产Nisin部分特性的研究

Study on lnhibitive Characteristics of Native Nissin

研究温度、 pH、蛋白酶、保存条件对国产 Nisin抑菌活性的影响，以及抑菌谱等。结果表明温度、 pH会显著影响 Nisin活性，在酸性条件下， Nisin对温度较稳定，随 PH值的增加，温度越高， Nisin活性下降越显著。温度越低越有利于保存，干制品比溶液易保存。国产 Nisin对胃蛋白酶不敏感，但对胰蛋白酶敏感。国产 Nisin对革兰氏阳性细菌有抑制作用，而对革兰氏阴性细菌、霉菌和酵母没有作用。

Experiments were conducted to study the effects of temperature,pH,protease and storage condition on the activity of native nisin through the inhibitory spectrum.The results indicated that nisin activity was affected significantly by temperature and pH.Under acidic condition nisin was stable to temperature.But as pH increased,the higher the temperature,the more the losses of nisin activity.The lower temperature was of advantage to nisin storage.Storage life of dry nisin was better than its solution.It was observed that native nisin was sensitive to trypsin,but insensitive to pepsin.The results of inhibitory spectrum experiments revealed that native nisin was inhibitive against Gram－ positive bacterium,but not inhibitive on Gram－ negative bacterium,yeast or mold.