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β-酪啡肽-7的多克隆抗体的制备 被引量:2

Preparation of β-Casomorphin-7’s polyclonal antibodies
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摘要 β-酪啡肽-7 经戊二醛与牛血清白蛋白(BSA)交联后的复合物免疫伊莎蛋鸡5只。6个月后,对鸡血清、所产蛋的卵黄和卵黄氯仿抽提上清液进行琼扩效价分析。结果表明,血清抗体的最高效价达到1∶8,后两者的效价为1∶4。 β-Casomorphin-7 was covalently attached to BSA,the β-Casomorphin-7/BSA conjugation was used to immunized 5 Yisa layers for 6 months.Hens' serum,the yolk and the water phase of CHCl3-extract of Yolk(WCEY) were collected for agar gel immunodiffusion test.The result show that the highest serum antibodies,titre is up to 1∶8,the yolk and the WCEY's highest antibodies's titre is up to 1∶4.
机构地区 南京农业大学
出处 《中国乳品工业》 CAS 北大核心 2001年第2期10-11,共2页 China Dairy Industry
关键词 β-酪啡肽-7 伊莎蛋鸡 免疫扩散实验 多克隆抗体 制备 酪蛋白 水解 β-Casomorphin-7 Yisa laysers immunodiffusion test polyclonal antibodies
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