小麦Rubisco的纯化、鉴定及其活性测定

Purification and Identification of Rubisco in Wheat

以小麦叶片为材料 ,根据Rubisco的溶解度、分子大小和形状、电荷种类等特性采用盐析、凝胶过滤、离子交换层析技术进行该酶的分离、纯化。纯化的Rubisco经聚丙烯酰胺凝胶电泳鉴定呈一条谱带 ,经SDS -聚丙烯酰胺凝胶电泳呈 2条谱带 ,一条带为Rubisco大亚基 ,分子量 5 5 0 0 0左右 ;另一条为Rubisco小亚基 ,分子量 14 80 0。

Rubisco was separated and purified from wheat leaves by ammonium sulfate precipitation, gel filtration, ion-exchange chromatography on the basis of Rubisco characteristic, such as dissolvability, molecular size and shape,and electric charge. The pure Rubisco showed only on band by PAGE-electrophoresis and two predominant bands by SDS-PAGE electrophoresis. One was the large subunit, whose molecular weight was about 55000 and the other was the small subunit, whose molecular was about 14 800.