p27kipl和cyclin D1在肾细胞癌中的表达及生物学意义

Expression of p27kipl protein and cyclin D1 protein in renal cell carcinoma

目的 探讨细胞周期蛋白依赖性激酶抑制蛋白p2 7kipl(p2 7)和细胞周期蛋白D1(CyclinD1)与肾癌生物学行为的关系。 方法 采用免疫组化SP法测定 41例肾透明细胞癌及 2 1例正常肾组织石蜡切片中p2 7蛋白和CyclinD1蛋白的表达。 结果 p2 7在正常肾组织中的阳性率为19 .0 % ,在肾细胞癌组织中的阳性率为 5 8.5 % (P <0 .0 1)。p2 7在G1、G2 、G3 级肾癌组织的阳性率分别为 82 .4%、5 7.1%、2 0 .0 % ,p2 7表达与肾癌的分级呈负相关 (P <0 .0 1)。CyclinD1在正常肾组织中的阳性率为 33.3% ,在肾癌组织中的阳性率为 6 8.3% (P <0 .0 1)。CyclinD1在G1、G2 、G3 肾癌组织的阳性率分别为 88.2 %、71.4%、30 .0 % ,CyclinD1蛋白的表达与肾癌的分级亦呈负相关 (P <0 .0 5 )。p2 7和CyclinD1间在肾癌中的表达呈正相关 (P <0 .0 1) ,r =0 .6。 结论 p2 7和CyclinD1与肾癌的组织学分级呈负相关 ,提示 p2 7和CyclinD1有可能作为判别肾癌分化程度的标志物 ,并可能成为判断预后的有用指标。

Objective To investigate the expression of p27 protein and cyclin D1 protein in human renal cell carcinoma and in normal kidney cortex tissue,and to explore the correlation of these proteins expression with biologic behavior of tumors. Methods p27 and cyclin D1 expression were studied in formalin-fixed,paraffin-embedded sections of renal cell carcinoma and in normal kidney cortex tissue by means of a specific and sensitive immunohistochemical S-P assay. Results The positive rates of p27kipl protein and cyclin D1 protein in renal cell carcinoma were significantly higher than the positive rates in normal kidney cortex tissues (P<0.01),and p27kipl and cyclin D1 were concurrently expressed in RCC.There was an inverse relationship between the expression rate of p27kipl,cyclin D1 and the histological tumor grades (P<0.01). Conclusions p27kipl and cyclin D1 expression rates are negatively correlated with the malignant degree of RCC,indicating that p27kipl and cyclin D1 might be an useful prognostic marker in renal cell carcinoma.