摘要
目的观察视网膜色素上皮(RPE)细胞中,IL-6的表达及地塞米松对IL-6表达的抑制作用。方法培养的人RPE经IL-1β(10μg/L)刺激及地塞米松作用后,采用ELASA、免疫组化和原位杂交等方法,检测培养的人RPEIL-6mRNA及其蛋白的表达。结果用IL-1β刺激后8h,RPE细胞表达的IL-6约为2000ng/L·106细胞;地塞米松可明显抑制IL-6蛋白的产生(200ng/L·106细胞),与对照组相比较差异显著(P∨0.01)。免疫组化染色显示,表达的IL-6在RPE细胞胞浆中呈浓淡不一的棕黄色,地塞米松作用组染色较浅。原位杂交表明,IL-6mRNA在RPE细胞胞浆中的表达呈浓淡不一的紫蓝色,地塞米松作用组染色与对照组相同。图像分析显示,二者的吸光度(A)值无明显差别(P∧0.05)。结论在IL-1β刺激下,人RPE细胞可表达IL-6的mRNA及其蛋白,地塞米松能有效地抑制RPE细胞中IL-6蛋白的表达。
Aim To investigate IL 6 expression in retinal pigment epithelium(RPE) cells and inhibitory effect on its expression by dexamethasone. Methods Cultured RPE cells was treated with IL 1β(10 μg/L) and dexamethasone. Expressions of IL 6 mRNA and its protein were detected by ELISA, immunohistochemical staining and in situ hybridization(ISH)assay. Results IL 6 in conditioned media was 2 000 ng/L·106 cells after IL 1β stimulation for 8 h. Dexamethasone could inhibit significantly IL 6 production (200 ng/L·106 cells, P∨0.01). Immunohistochemical staining showed light brown staining in RPE cells expressing IL 6 after stimulation with IL 1β. Dexamethasone treated RPE cells were stained lightly. However, ISH displayed that there was no marked difference in mRNA expression between IL 1β and dexamethasone treated groups. Conclusion Cultured human RPE cells stimulated with IL 1β significantly accelerate IL 6 production, while dexamethasone is an effective inhibitor of IL 6 production.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第3期260-262,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助
No.39970780
