HBV阳性血清诱导人外周血单个核细胞凋亡的检测及意义

Apoptosis of peripheral blood mononuclear cells induced by the HBV positive serum and its significance

目的研究乙型肝炎病毒(HBV)对外周血单个核淋巴细胞(PBMC)凋亡的作用。方法利用10名健康献血员外周血分离PBMC,分为加HBV阳性血清组和加健康人血清对照组,经培养72h后,采用PI染色法在流式细胞仪上检测细胞的凋亡情况。结果加HBV阳性血清组培养细胞的凋亡率为(39.56±7.03)%,明显高于加健康人血清组培养细胞的凋亡率(27.57±7.78)%,两组间具有非常显著的差异(P∨0.02)。结论HBV可能具有诱导PBMC凋亡的能力,这可能是形成HBV慢性感染免疫耐受的一个重要机制。

Aim To appraise the role of HBV in apoptosis of peripheral blood mononuclear cells(PBMCs). Methods PBMCs were isolated from 10 healthy blood donors. PBMCs from one blood donor were devided into two samples. One sample was cultured with the HBV positive serum. Another sample was cultured with the serum of healthy person. After 72 hours incubation,the cells were harvested and stained with PI. Then apotosis of the PBMCs was assayed by flow cytometry. Results Apoptotic rate of PBMCs incubated with HBV positive serum(39.56±7.03)% was significantly higher than that with the serum of normal person(27.57±7.78)%(P∨0.02). Conclusion HBV can induce apoptosis of PBMCs. It may be an important mechanism of peripheral immune tolerance in HBV chronic infection.