摘要
目的鉴定自制重组人血管生长素(rhANG)及其单克隆抗体(mAb)。方法采用凝胶扫描法分析rhANG的纯度;ELISA等方法检测抗rhANGmAb的特异性,鸡胚绒毛尿囊膜实验检测rhANG及其mAb的生物学活性,并与商品化标准品的免疫结合性进行对比研究。结果rhANG的纯度达96%,具有促进血管生长的生物学活性;抗ANGmAb具有很好的特异性和抑制血管生长的活性;而且二者较标准品均具有良好的免疫结合活性。结论该rhANG及其mAb可用于进一步的实验和临床应用研究。
Aim To detect the biological activity of the home made recombinant human angiogenin and its monoclonal antibody(mAb). Methods Gel scanning was used to analyze the purity of home made rhANG. ELISA and chicken CAM assay were used to detect the specificity and biological activity of ANG and anti ANG mAb, respectively. Immunobinding activities of home made rhANG and the standard rhANG were compared by routine ELISA. Results The purity of home made rhANG reached 96% and could induce the angiogenesis. The anti ANG mAb showed high specificity and inhibited effectively the proliferation of blood vessels induced by angiogenin. The home made and standard ANG, both products possessed good immunological binding activity. Conclusion The home made rhANG and its mAb can be used for further basic and clinical research.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第3期277-279,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
军队自然科学基金资助
No.96D0331
广东省自然科学基金资助
No.960668.
