同型半胱氨酸对大鼠主动脉内皮细胞生长和功能的影响

Effects of homocysteine on cultured rat aortic endothelial cells

Objective To study the possible mechanism of the effects ofhomocysteine on the formation of atherosclerosis (AS). Methods Aortic endothelial cells of SD rat were cultured and treated with a concentrated homocysteine for 24h,the cultured cells and the contents in the medium were studied by MTT colorimetric assay,flow cytometric method (FCM),PGI２,thromboxane A2,(TXA２),nitric oxide (NO),endothelin(ET) in the contents were examined. Results (1)homocysteine had an inhibitory effect on the proliferation of rat aortic endothelial cells;(2)homocysteine prevented rat aortic endothelial cell form G1-phase into S-phase in DNA synthesis;(3)vascular relaxing factor NO,PGI2 content in culture media in homocysteine group was significantly lower than that of control group;(4)vasoconstrictors-TXA2,ET were markedly higher than that of control group;(5)ox-LDL which had a bad effect on endothelial was higher than that of control group. Conclusions In the experimental model study,homocysteine was proved having a definite harmful effect on the aortic endothelial cells and this initial damage might play a very important role in the process of AS formation.