摘要
目的 探讨硒 (Se)抗石英尘的细胞毒作用及其机理。方法 采用体外细胞培养法 ,观察在不同浓度硒作用下 ,染石英尘的肺泡巨噬细胞 (AM)膜脂质过氧化物丙二醛 (MDA)生成量和AM内抗氧化酶 -超氧化物歧化酶 (SOD)活性。结果 在体外染石英尘中加入硒有效抑制AM膜丙二醛生成量 ,其中以 1 0× 10 -4 mol/L硒作用最为明显 ,并可提高AM内超氧化物歧化酶活性 ,且有一定的剂量反应关系。
Objective To study the effects and mechanism of selenium on the injury of alveolar macrophage(AM) exposed in vitro to quartz.Methods The different malondialdehyde(MDA)quantity and superoxide dismutase(SOD)activity were determined after AM exposed in vitro to quartz and different dose of selenium.Results Selenium could inhibit the production of MDA in AM. 1 0×10 -4 mol/L selenium had the most obvious inhibitory effect.Selenium could also increase the activity of SOD in AM with an obvious dose response relationship.Conclusion Selenium could antagonize the lipid peroxidation of alveolar marophage by quartz.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第5期414-415,共2页
Chinese Journal of Public Health
关键词
硒
肺泡巨噬细胞
脂质过氧化
抗氧化酶
实验研究
quartz
selenium
alveolar macrophage
lipid peroxidation
antioxidant enzyme