糖尿病大鼠肾、胰基因组DNA甲基化状态的变化

THE CHANGES OF GENOME DNA METHYLATION OF KIDNEY AND PANCREAS FROM DIABETIC RAT

从正常大鼠、糖尿病大鼠、中药糖微康治疗的糖尿病大鼠、西药开博通治疗的糖尿病大鼠的肾、胰等组织中提取出其基因组DNA ,应用对DNA甲基化作用敏感的限制性核酸内切酶HpaⅡ和MspⅠ (为一组同裂酶 )进行了限制性酶切图谱分析 ,结果表明 ,DNA甲基化作用的主要位点在CpG二核苷酸处 ,且“糖微康”在关闭患糖尿病后肾组织中被异常活化的基因的同时 ,还能活化糖尿病动物胰组织中处于静息状态的基因 .由此可见糖尿病的发病和治疗都与DNA甲基化作用密切相关 .

The genome DNA of kidney and pancreas is prepared from control animals, diabetic animals, diabetic animals treated with the traditional medicine——Tang Wei Kang, and diabetic animals treated with Captopril which is one of the angiotensin-convering enzyme inhibitors. Then the genome DNA is cleaved by two restriction enzymes HpaⅡ and MspⅠ which are sensitive to DNA methylation condition. The results indicate that CpG is the main site of DNA methylation in the genome. Tang Wei Kang can inhibit some genes expression in the kidney and provoke some gene into transcript condition in the pancreas. It suggests that DNA methylation plays a key role during the process of diabetes developing and curing.