SEDSS/异辛烷/水反胶束体系对Cyt-C的提取

Extraction of cytochrome C with SEDSS/iso-octane/water reversed micellar system

报道了在SEDSS/异辛烷 /水反胶束体系中相转移提取细胞色素C的最佳条件 .结果表明 ,在室温下 ,pH值为 2～ 6,离子强度为 0 0 7～ 0 .2 5mol·L- 1及表面活性剂的浓度大于 60mmol·L- 1的条件下 ,提取率可达 99%以上 ;pH =1 2 .2、离子强度为 0 5mol·L- 1及乙醇体积分数为 50 %的条件下 ,反提取率可达 57% ,正提和反提时都无界面膜状物产生 .

This paper reports the best condition of the phase transfer extraction of cytochrome C in SEDSS/iso-octane/water reverse micellar system. The results indicated that the extraction ratio is above 99% in the pH range 2 to 6, ionic strength equal 0.07 to 0.25 mol·L -1 , concentration of surfactant is above 60 mmol·L -1 , the back extraction ratio is above 57% in the pH is 12.2, ionic strength equal 0.5 mol·L -1 ,concentration of ethanol ( V Ethanol / V Water ) is 50%, no undissolved substance on the interface, and need not co-surfactant.