蓖麻蚕血细胞的体外培养及病毒感染

CELL CULTURE AND VIRAL INFECTION OF HEMOCYTES OF THE ERISILKWORM, PHILOSAMIA CYNTHIA RICINA

细胞在加有适量的植物凝血素(PHA)的TC-100和Grace培养基中生长情况要比在不加PHA的要好,尤其是在加有PHA的TC-100培养基中,细胞增殖尤为明显,许多团形细胞形成增殖中心,并且增殖较快,大约10天左右即基本长满瓶底进行第一次传代培养,原代培养的细胞主要有两种类型:园形细胞和梭形细胞。传代培养细胞主要是园形细胞。多次实验结果表明,PHA对昆虫血淋巴中的某一类型细胞具有刺激增殖作用。笔者对这种现象进行了初步的讨论,用蓖麻蚕核型多角体病毒感染上述培养的细胞,通过光镜和电镜观察呈现出典型的细胞病理变化。

This paper deals with the growth of hemocytes of the eri-silkworm, Philosamia cynthia ricina in cultures. The cells division speed up and formed centres of multiplication when medium TC-100 was aupplmented with appropori ate amount of phytoagglutinin (PHA). In primary cultures, it consists of round and spindieshaped cells,and while in sub-cultures the cells were mainly in round shape. The experiments conducted clearly demon-strated that the PHA could stimulate the multeplication of insect hemocytes as well as that of mammalian lymphocytes. When the cultured cells challenged with nuclear polyhedrosis virus of the eri-silk-worm(Pcr-NPV),most cells were heavily infected and the typical cytopathological changes were seen under light microscopy and electron microscopy.