摘要
应用基因枪法对超级杂交水稻培矮-64S进行了转化。质粒载体pKC-3串联了三个抗虫基因,将其转化成熟胚诱导的愈伤组织,共获得33株转基因植株。分别对不同的基因进行了PCR分析,结果表明,三个抗虫基因均已整合到水稻基因组。
Transformation of two-line genetic male sterile indica rice variety Pei'ai 64S was conducted by particle bombardment. Three insect-resisting genes ligased into plasmid vec- tor pKC-3 was transformed into mature embryo calli and a totle of xx transgenic plants had been obtained. The PCR analysis of each different gene showed the integration of three in- sect-resisting genes into the genome of transgenic plants.
出处
《中山大学研究生学刊(自然科学与医学版)》
2001年第1期14-17,共4页
Journal of the Graduates Sun YAT-SEN University(Natural Sciences.Medicine)