摘要
采用透出液在体外和细菌（表皮葡萄球菌和大肠杆菌）一起孵育的方法，以观察透析液在不同条件下对细菌生长的影响。发现在４℃条件下，透出液对细菌生长的影响不大，但在３７℃条件下，透出液中大肠杆菌数则显著减少（Ｐ＜０．０５）；当用细胞冻融破坏透出液中的细胞后，表皮葡萄球菌和大肠杆菌数均显著增加（Ｐ＜０．０１），特别是在含５％ＣＯ２时，新鲜透析液虽支持大肠杆菌的生长，但却抑制表皮葡萄球菌的繁殖。本研究结果提示，透出液中细菌的生长受温度、ＣＯ２、透出液中吞噬细胞及透析液在体内停留的时间等因素影响，特别是透出液中的吞噬细胞。若破坏透出液中的吞噬细胞，有助于提高透出液的细菌培养阳性率。
he effect
of dialysis on recovery of bacteria under different conditions was studied by incubating
dialysis with S.epidermidis(SE)and E.coli(EC)in vitro.Dialysis didn't affect the number of
bacteria in 4℃,but significantly decreased the number of EC in 37℃.If the phagocytes in
effluent was disrupted by freeze thaw cycle,the number of SE and EC was significantly
increased,especially incubated with 5% CO2.Although fresh dialysate supported the growth of
EC ,it suppressed the growth of SE.It suggests that the field of bacteria from dialysis effluents
is influenced by temperature,CO2, phagocytes in effluents and the dwell time of dialysis and so
on.The phagocytes are the most important.Disrupting the phagocytes in dialysis effluents may
increase the field of positive culture results.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1994年第1期34-36,共3页
Chinese Journal of Nephrology,Dialysis & Transplantation
