6A8 α-甘露糖苷酶基因的mRNA表达与染色体定位

Chromosomal localization and mRNA expression of 6A8 α-mannosidase gene

目的 对 6A8α 甘露糖苷酶基因作染色体定位 ,比较 6A8α 甘露糖苷酶mRNA在人多种免疫细胞株的表达。方法 用Fish原位杂交作染色体定位 ,用RT PCR检测mRNA表达。结果 6A8α 甘露糖苷酶基因定位于人第 13号染色体长臂的 31～ 32区。 6A8α 甘露糖苷酶mRNA在B细胞株SKW 6高表达 ,在B细胞株 3D5、BJAB、CESS、Daudi、Nalm 6中度表达 ,在B细胞株Navalm ,T细胞株GM、Jurkat,组织细胞瘤细胞株U937弱表达 ,在T细胞株Peer,慢性髓性白血病细胞株K5 6 2极弱表达。结论 6A8α 甘露糖苷酶基因定位于人第 13号染色体长臂的 31～ 32区 ,其mRNA表达水平在各种人免疫细胞株不同。

Objective To localize 6A8 α-mannosidase gene in chromosome and to compare the 6A8 α-mannosidase mRNA expression in various human immune cell lines. Methods Fish and RT-PCR. Results 6A8 α-mannosidase gene is localized in human chromosome 13q31-32. 6A8 α-mannosidase mRNA shows a high expression in B cell line SKW 6, a median expression in B cell line 3D5, BJAB, CESS, Daudi, Nalm 6, a weak expression in B cell line Navalm, T cell line GM, Jurkat and histocyte line U937, and a very weak or no expression in T cell line Peer and a chronic myelogenous leukemia cell line K562. Conclusion 6A8 α-mannosidase gene is localized in human chromosome 13q31-32 and the expression of 6A8 α-mannosidase in mRNA level is variant in different human immune cell lines.