bFGF对缺血性大鼠脑细胞凋亡及相关基因表达的影响

The effect of bFGF on the apoptosis and its relative gene in rat brain following cerbral ischemia-reperfusion

目的 研究外源性碱性成纤维细胞生长因子（ｂＦＧＦ）对脑缺血再灌流后 ＨＳＰ ７０蛋白、ｐ５３基因表达及细胞凋亡的影响。方法 线栓法制成大鼠大脑中动脉闭塞及再灌流模型，用原位杂交、免疫组化及原位末端标记的方法观察大鼠大脑中动脉闭塞２ｈ后再通ｏｈ～７２ｈ脑组织ＨＳＰ７０蛋白、ｐ５３基因的表达及凋亡细胞的分布，侧脑室注射外源性ｂＦＧＦ对它们的影响。结果ｂＦＧＦ组与生理盐水组相比于６ｈ～４８ｈ各时间点的ＨＳＰ ７０蛋白表达增强；ｐ５３表达减弱；凋亡细胞数明显减少（Ｐ＜０.０５，Ｐ＜０．０１）。结论外源性ｂＦＧＦ能抑制脑缺血再灌流后细胞凋亡并调控其相关基因的表达。

AIM To study the effect of exogenous bFGF on the cell apoptosis and the expression of HSP 70 protein and p53 gene. METHODS The effect of exogenous bFGF on HSP 70 protein and gene expression was examined vial insitu hybridization and immunohistochemistry, at 0-72 h reperfusion after the middle cerebral artery (MCA) was occluded for 2 h in rats. Simutaneously, the distribution of apoptosis was observed. RESULTS The expression of HSP 70 protein elevated and the expression of p53 gene and cell apoptosis decreased in bFGF treated rats as compared with ischemia rats. (P < 0.05, P < 0.01 ). CONCLUSION Exogenous bFGF can supress the apoptosis and regulate its relative gene.