牛磺酸对阿霉素致膈肌毒性的保护作用

Protective effect of taurine on diaphragm against adriamycin cytotoxicity

目的研究牛磺酸对阿霉素致隔肌毒性的保护作用。方法家兔１８只，分３组：①对照组：静脉注射生理盐水（ＮＳ）×５ ｄ；②ＮＳ＋阿霉素组：静脉注射ＮＳ×５ ｄ；③牛磺酸＋阿霉素组：静脉注射牛磺酸 １００ ｍｇ·ｋｇ－１ × ５ ｄ。 ２、３组于末次注射后 ２ ｈ，静脉注射阿霉素 １０ ｍｇ·ｋｇ－１组注射等量ＮＳ。２４ｈ后，麻醉动物，测量跨膈压（Ｐｄｉ）、膈肌诱发电位（ＤＥＰ）。同时测定膈肌组织中丙二醛（ＭＤＡ）含量和超氧化物歧化酶（ＲＪＤ活性。电镜观察细胞器改变。结果阿霉素（ １０ ｍｇ· ｋｇ－１）可使跨膈压（ Ｐｄｉ）、膈肌诱发电位（ＤＥＰ）幅度降低（ Ｐ＜ ０． ０５）；膈肌中 ＭＤＡ含量增加， ＳＪＤ活性降低（Ｐ＜０．０５），肌小节和线粒体形态改变。而牛磺酸１００ｍｇｋｇ－１可抑制上述现象。结论牛磺酸对阿霉素致膈肌毒性有保护作用。

AIM To study protective effect of taurine on diaphragm against adriamycim cytotoxity in rabbits. METHODS The rabbits were divided randomly into three groups. groups group: The animals were administered NS 2 ml.kg- 1 for five days. ②Adriamycin + NS group: The animals were administered NS 2ml. kg-1 for five days and then administered adriamycin 10 mg. kg- 1. ③Adriamycin + taurine group: The animals were administered taurine 100 mg. kg-1 for five days and then administered adriamycin 10 mg·kg- 1. After 24 h, three groups animals were anaesthetized. The transdiaphragmatic pressure(Pdi) and diaphragm evoked potential(DEP) were measured. an and MDA were tested. the ultrastructure of diaphragm cells was investigated by eletronmicroscopy. RESULTS The Pdi and amplitude of DEP were lowered by adriamrcin(P<0 .01 ). The levels of MDA was increased and the activity of SOD was decreased in diaphragm (P < 0.05 ). The disorder of myofibrills, swelling of mitochondria with broken cristase can be observed by electronmi- croscopy. The above changes were inhibited by taurine. CONCLUSIONS The taurine could scavenge the toxic radicals generated by adriamycin and protect the diaphragm myocytes.